Current Proteomics, Volume (16), No (3), Year (2019-2) , Pages (188-198)

Title : ( Assessment of Signal Peptides to Optimize Interleukin 2 -IL-2- Folding and Expression )

Authors: narges nazifi , Mojtaba Tahmoorespur , Mohammad Hadi Sekhavati , Alireza Haghparast , Mohammad Ali Behroozikhah5 ,

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Background: Using a bacterial expression system such as Escherichia coli -E. coli- is very common for protein expression because of its simplicity, low cost and high efficiency. Objective: In order to express proteins that contain di-sulfide bands, an oxidative space such as the periplasmic environment of the bacteria is required. Therefore, a leader sequence which named Signal Peptide -SP- is needed to direct recombinant protein to fold in periplasmic space. Interleukin-2 -IL-2- is a prominent cytokine which known as growth factor for T-cells and typically produced by a variety of immune cells that stimulate and regulate inflammatory and immune responses. Methods: This study was designed to predict the best signal peptides to express IL-2 in E. coli. To predict the best signal peptides for the expression of IL-2 in Gram-negative bacteria -E. coli-, forty-five sequences of SPs were extracted from data base. Some most important details such as n, h and c regions of signal peptides and their probability were studied through the signalP software. Afterwards, physico–chemical features of SPs were analyzed by Portparam and Solpro tools. Finally, secretion-pathway and sub-cellular localization sites were evaluated by PRED-TAT and ProtcompB softwares. Results: At the end of the in-silico analyzes, it was determined that ccmH, PelB, traU, yohN, lolA, yhcN are the most reliable SPs, respectively, with highest score and best performing to express the IL-2 protein in E. coli.


, Signal peptide, IL-2, E. coli, periplasm, interleukin, T-cells, immune cells
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author = {Nazifi, Narges and Tahmoorespur, Mojtaba and Sekhavati, Mohammad Hadi and Haghparast, Alireza and محمدعلی بهروزی خواه},
title = {Assessment of Signal Peptides to Optimize Interleukin 2 -IL-2- Folding and Expression},
journal = {Current Proteomics},
year = {2019},
volume = {16},
number = {3},
month = {February},
issn = {1570-1646},
pages = {188--198},
numpages = {10},
keywords = {Signal peptide; IL-2; E. coli; periplasm; interleukin; T-cells; immune cells},


%0 Journal Article
%T Assessment of Signal Peptides to Optimize Interleukin 2 -IL-2- Folding and Expression
%A Nazifi, Narges
%A Tahmoorespur, Mojtaba
%A Sekhavati, Mohammad Hadi
%A Haghparast, Alireza
%A محمدعلی بهروزی خواه
%J Current Proteomics
%@ 1570-1646
%D 2019