Polyphylla adspersa Motschulsky -Coleoptera: Melolonthidae- is the key white grub species in the Eastern part of Iran. Due to increasing concerns about its damage on different tree and crop species, addressing environmentally friendly tactics including biological control has top priority. Here, we report new natural enemies of the white grub larvae in Razavi Khorasan province, North East Iran. The most prevalent natural enemies were entomopathogenic fungi including, Beauveria bassiana s.l -Hypocreales: Cordycipitaceae- and Metarhizium anisopliae s.l -Hypocreales: Clavicipitaceae-. A parasitic nematode, Cephalobellus sp. -Oxyurida: Thelastomatidae- as well an insect pathogenic nematode, Heterorhabditis bacteriophora -Rhabditida: Heterorhabditidae- were collected as natural entomopathogens. Among protistans, two species of eugregarines, Stictospora sp. and Gregarina sp. were found as coelomic parasite, while infected the larvae. A tachinid fly, Microphthalma europaea -Diptera: Tachinidae- was identified as koinobiont endoparasitoid of the larva and a humpbacked fly, Megaselia scalaris -Diptera: Phoridae- recognized as an ectoparasite. All records are presented as first association with the P. adspersa. New information about all newly recorded taxa is provided here. This information is useful for designing future biological control programs.

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