زیست شناسی کاربردی, دوره (31), شماره (2), سال (2018-9) , صفحات (137-155)

عنوان : ( ساخت و آنالیز بیان ناقل جفتی گیاهی pCaBGi - (Construction and expression analysis of plant binary vector pCaBGi) )

نویسندگان: فرهاد شکوهی فر , ناهید عباسپور , صهبا طوسی , نیره سادات غفارینیا ,
فایل: Full Text

استناددهی: BibTeX | EndNote

چکیده

Transient expression in the plant leaf cells is a relatively fast technique to analyses regulatory sequences. To gain the approach advantages, a convenient expression vector would be needed for transient analysis of regulatory elements. In this study, to construct such a vector we used pBGi which provides the regulatory elements cloning site::minimal promoter as insert and pCAMBIA3301 that after removing the CaMV 35S promoter was used as backbone. The new vector, pCaBGi was constructed by replacing BamHI/SnaBI fragment (cis-acting cloning site::Minimal promoter) of pBGi with a BamHI/SnaBI fragment (CaMV 35S promoter) isolated from the pCAMBIA3301. The recombinant colonies were screened using colony PCR with and accuracy of the construction steps has been confirmed by sequencing using PSh4-F2/R specific primers. GUS assay showed that the intron containing GUS gene do not expressed agrobacterium strain GV3101 as a prokaryotic system. Injection of GV3101 harboring pCaBGi in tobacco leaves showed very low Basal expression of minimal promoter. The Sequence of pCaBGi has been submitted to gene bank using sequin software with the Accession number MG719235.

کلمات کلیدی

, Binary vector, vector construction, Transient expression, Agroinjection, synthetic promoter analysis
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@article{paperid:1074755,
author = {شکوهی فر, فرهاد and ناهید عباسپور and طوسی, صهبا and نیره سادات غفارینیا},
title = {ساخت و آنالیز بیان ناقل جفتی گیاهی pCaBGi - (Construction and expression analysis of plant binary vector pCaBGi)},
journal = {زیست شناسی کاربردی},
year = {2018},
volume = {31},
number = {2},
month = {September},
issn = {2251-7901},
pages = {137--155},
numpages = {18},
keywords = {Binary vector; vector construction; Transient expression; Agroinjection; synthetic promoter analysis},
}

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%0 Journal Article
%T ساخت و آنالیز بیان ناقل جفتی گیاهی pCaBGi - (Construction and expression analysis of plant binary vector pCaBGi)
%A شکوهی فر, فرهاد
%A ناهید عباسپور
%A طوسی, صهبا
%A نیره سادات غفارینیا
%J زیست شناسی کاربردی
%@ 2251-7901
%D 2018

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