Journal of Agricultural Science and Technology, ( ISI ), Volume (22), No (4), Year (2020-6) , Pages (989-998)

Title : ( Development and Validation of a Real-Time PCR Assay for the Identification of a Common Agrotis Species: Agrotis segetum (Lepidoptera: Noctuidae) )

Authors: pari naz matlab dokht , Lida Fekrat , Mohammad Zaki AGhl , Gholamhossein Moravvej , Asghar Shivani ,

Access to full-text not allowed by authors

Citation: BibTeX | EndNote

Abstract

The common cutworm, Agrotis segetum, is a serious soil pest of many vegetable and field crops all over the world. Morphological identification of Agrotis species is predominantly performed on adults ascribed to the deficiency of adequate identification keys for immature stages. In international trade, the immature life stages are frequently being intercepted at point of inspection, challenging the possibilities of morphological identification. To realize a rapid and reliable identification for all stages of A. segetum, a TaqMan real-time polymerase chain reaction (PCR) was developed based on the mitochondrial cytochrome oxidase I (COI) gene. All specimens of A. segetum (including various life stages) were detected and no cross-reactivity was observed with 5 nontarget Agrotis species in the specificity tests. The tests showed to be repeatable, reproducible and robust. The assay performed equally well with crushed insects and purified DNA, so the efficiency was added by removing DNA extraction step. The method has proven to be suitable tools for routine identification of all life stages of A. segetum considering the speed, specificity as well as sensitivity of the assay.

Keywords

, Agrotis segetum, qualitative real-time PCR, mitochondrial cytochrome oxidase I
برای دانلود از شناسه و رمز عبور پرتال پویا استفاده کنید.

@article{paperid:1076127,
author = {Matlab Dokht, Pari Naz and Fekrat, Lida and Zaki AGhl, Mohammad and Moravvej, Gholamhossein and Asghar Shivani},
title = {Development and Validation of a Real-Time PCR Assay for the Identification of a Common Agrotis Species: Agrotis segetum (Lepidoptera: Noctuidae)},
journal = {Journal of Agricultural Science and Technology},
year = {2020},
volume = {22},
number = {4},
month = {June},
issn = {1680-7073},
pages = {989--998},
numpages = {9},
keywords = {Agrotis segetum; qualitative real-time PCR; mitochondrial cytochrome oxidase I},
}

[Download]

%0 Journal Article
%T Development and Validation of a Real-Time PCR Assay for the Identification of a Common Agrotis Species: Agrotis segetum (Lepidoptera: Noctuidae)
%A Matlab Dokht, Pari Naz
%A Fekrat, Lida
%A Zaki AGhl, Mohammad
%A Moravvej, Gholamhossein
%A Asghar Shivani
%J Journal of Agricultural Science and Technology
%@ 1680-7073
%D 2020

[Download]