15th congress on stem cell biology and technology , 2019-08-28

Title : ( Donor Affect Tenogenic Differentiation of Equine Adipose Mesenchymal Stem Cells )

Authors: fatemeh younesi soltani , Abbas Parham , Hojjat Naderi Meshkin ,

Citation: BibTeX | EndNote

Abstract

Background:The advent of regenerative medicine has brought us the opportunity to regenerate, modify and restore organs function.Stem cells,a key resource in regenerative medicine, can generate into one or more specializedcelltypesliketenocytes.Beforeclinicalapplicationofstemcells,theircharacteristicsshouldbedetermined.Thisstudydesignedtoinvestigate weatherdonorhaseffectontenogenicdifferentiationofequine adiposemesenchymalstemcells(ASCs)inresponsetoplateletderivedgrowth-factor(PDGF). Materials and Methods: Equine ASCs derived from three horseswereusedinthisexperiment.ASCsatpassage5were culturedwithbasicculturemediumcontainingPDGF-BB(20 ng/ml),L-Prolinandascorbicacid(AA)at37°Cunder5%CO2 for14days.Thecellsincontrolgroup,wereculturedwithonly basicculturemedium.Attheend,SiriusRedandH&Estainingwereusedtoassesscollagenproduction,asaextracellular matrixproteinfortendon,andcellmorphology. Results:ASCsintreatmentgroupswerechangedtoelongated spindleshapeliketenocytes.TheresultsofSirusRedstaining revealedthattheproductionof collagenhasbeen elevatedin treatment groups comparedwith control groups signi9cantly. Statistical analysis showed thattheproduction of collagenis differentamongASCsderivedfrom3horses. Conclusion:Ourdatarevealedthatcelldifferentiationtoward tenocyteswasdifferentforeachhorses.Inotherwords,donor affecttenogenicdifferentiationpotentialofequineAscs.

Keywords

, Equine, Mesenchymal Stem Cells, Donor, Tenogenic Differentiation
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@inproceedings{paperid:1076509,
author = {Younesi Soltani, Fatemeh and Parham, Abbas and حجت نادری مشکین},
title = {Donor Affect Tenogenic Differentiation of Equine Adipose Mesenchymal Stem Cells},
booktitle = {15th congress on stem cell biology and technology},
year = {2019},
location = {تهران, IRAN},
keywords = {Equine; Mesenchymal Stem Cells;Donor;Tenogenic Differentiation},
}

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%0 Conference Proceedings
%T Donor Affect Tenogenic Differentiation of Equine Adipose Mesenchymal Stem Cells
%A Younesi Soltani, Fatemeh
%A Parham, Abbas
%A حجت نادری مشکین
%J 15th congress on stem cell biology and technology
%D 2019

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