Title : ( Growth Differentiation Factor-6 (GDF-6) Induce Tenogenic Differentiation of Equine Adipose-Derived Mesenchymal Stem Cells (Eq-Ascs) )
Authors: shabnam javanshir , Abbas Parham , Hojjat Naderi Meshkin ,
Full TextAbstract
Background:Improvementofmusculoskeletaldifferentiation ofmesenchymalstemcells(MSCs)(bothandinvivo) isaninterestingeldsinstemcellresearch.Inordertousestem cells for treatment of orthopedic disorders especially tendon injuries,usingdifferentgrowthfactorsarerecommended.The aimofthis studywasto evaluate differentiation of eq-ASCs inresponsetogrowthdifferentiationgrowthfactor-6(GDF-6). Materials andMethods: Characterized andfrozen eq-ASCs from3horseswerethawedandwereculturedtopassage4(P4). Then,P4cellsweresubjectedtodifferentiationforaperiodof 14daysinaculturemediumcontainingtheGDF-6factor(20 ng/ml)astreatmentgroup.Incontrolgroup,mediumwasthe same astreatmentgroupexceptinducingfactors andGDF-6. After14days,tenogenicdifferentiationwasevaluatedbyH&E and Siriusredstainingforthemorphological assessment and measurement of collagenproduction.Inaddition,the expressionofspecicgenesincludingsclerosis(Scx),collagentype 1(ColIα1)andMohawkwereevaluatedusingqPCRmethod. Results:H&Estainingrevealedastretchingandspindleshape (tenocye-like)ofcellsinthetreatmentgroup.Siriusredstainingdatashowedasignicantincreaseincollagenproductionin treatmentgroupcomparedwithcontrolgroup.BasedonqPCR data, expression of Mkx, Scx and ColI was signicantly increasedinthetreatmentgroupcomparedtocontrolgroup. Conclusion:TheresultsofthisstudyshowedthatGDF-6could inducetenogenicdifferentiationineq-ASCs.
Keywords
, GDF-6, Mesenchymal Stem Cells, Tenogenic Differentiation, Horse
@inproceedings{paperid:1076510,
author = {Javanshir, Shabnam and Parham, Abbas and حجت نادری مشکین},
title = {Growth Differentiation Factor-6 (GDF-6) Induce Tenogenic Differentiation of Equine Adipose-Derived Mesenchymal Stem Cells (Eq-Ascs)},
booktitle = {15th congress on stem cell biology and technology},
year = {2019},
location = {تهران, IRAN},
keywords = {GDF-6;Mesenchymal Stem Cells;Tenogenic Differentiation;Horse},
}
%0 Conference Proceedings
%T Growth Differentiation Factor-6 (GDF-6) Induce Tenogenic Differentiation of Equine Adipose-Derived Mesenchymal Stem Cells (Eq-Ascs)
%A Javanshir, Shabnam
%A Parham, Abbas
%A حجت نادری مشکین
%J 15th congress on stem cell biology and technology
%D 2019
