Journal of Agricultural Science and Technology, ( ISI ), Volume (21), No (7), Year (2019-12) , Pages (1819-1830)

Title : ( Identification of Alkaline Protease Producing Bacilli from Sludge of Bactofuge and Separator Using Culture–Based and Molecular Techniques )

Authors: maryam tolou , Farideh Tabatabaei yazdi , Sayed Ali Mortazavi , Mohammad Reza Edalatian Dovom ,

Access to full-text not allowed by authors

Citation: BibTeX | EndNote

Abstract

Dairy factories produce high volume of sludge from bactofuge and separator. Meantime, global demand for the proteases is increasing. Recently, utilization and conversion of the waste materials into value added product is a sustainable process. The objective of this study was to investigate the potential of bactofuge and separator sludge to produce alkaline protease enzymes. Total viable aerobic and anaerobic counts were determined on Plate Count Agar at 37 and 50ºC for both types of sludge. Lactobacillus count in MRS Agar plates corresponded to 3.12±0.25 log CFU mL-1 for sludge of bactofuge and 3.085±0.2 log CFU mL-1 for sludge of separator. Mold and yeast had population levels of 2.3±0.1 log CFU mL-1 for bactofuge and 2.08±0.1 log CFU mL-1 for separator. Proteolytic bacteria were isolated from dairy sludge using Skim Milk Agar media. A clear zone of Skim Milk hydrolysis indicated protease-producing organisms. Different cultural parameters (temperature, pH, thermal shock, and kind of sludge) were optimized for maximal enzyme production. Maximum proteolytic activity was observed at 37◦C (P< 0.05). Isolated alkaline protease producing Bacilli were identified by Polymerase Chain Reaction (PCR). The species were identified as Bacillus cereus strain zk2, Bacillus sp. cp-h71, Bacillus thuringiensis strain ILBB224, and Bacillus sp. Bac6D2.

Keywords

, Bacillus, Dairy sludge, Polymerase Chain Reaction, Proteolytic activity
برای دانلود از شناسه و رمز عبور پرتال پویا استفاده کنید.

@article{paperid:1077420,
author = {Tolou, Maryam and Tabatabaei Yazdi, Farideh and Mortazavi, Sayed Ali and Edalatian Dovom, Mohammad Reza},
title = {Identification of Alkaline Protease Producing Bacilli from Sludge of Bactofuge and Separator Using Culture–Based and Molecular Techniques},
journal = {Journal of Agricultural Science and Technology},
year = {2019},
volume = {21},
number = {7},
month = {December},
issn = {1680-7073},
pages = {1819--1830},
numpages = {11},
keywords = {Bacillus; Dairy sludge; Polymerase Chain Reaction; Proteolytic activity},
}

[Download]

%0 Journal Article
%T Identification of Alkaline Protease Producing Bacilli from Sludge of Bactofuge and Separator Using Culture–Based and Molecular Techniques
%A Tolou, Maryam
%A Tabatabaei Yazdi, Farideh
%A Mortazavi, Sayed Ali
%A Edalatian Dovom, Mohammad Reza
%J Journal of Agricultural Science and Technology
%@ 1680-7073
%D 2019

[Download]