Title : ( Biosensing Applications of Graphitic Carbon Nitride-Chitosan Nanosheets on Glassy Carbon Electrode for Determination of Phytic Acid Biosensor )
Authors: Chakavak Esmaeili , Parviz Norouzi , Saiedeh Agh Atabay , Hossein Ahmadzadeh , Elaheh Kafshdare Goharshadi ,
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Abstract
A novel phytic acid (PA) biosensor was fabricated using phytase enzyme immobilized graphiticcarbon nitride (g-C3N4and chitosan (CH) on the surface of glassy carbon electrode (GCE). Theelectrochemical method used for determination of phytic acid was stripping cyclic voltammetry (CV)and differential pulse voltammetry (DPV). The synergistic effect of g-C3N4-CH/phytase made thebiosensor to possess the excellent activity toward the oxidation of the analyte in acetate buffer inpH 5.0. The biosensor exhibited a linear response to phytic acid as substrate from 5.0 to 25 ppmwith correlation confectioned (R2=097) and limit of detection (LOD) of 2.0 ppm phytic acid atoptimum pH 5.0 in 0.1 M acetate buffer. In addition, the biosensor excludes interfering substance,which makes the biosensor possesses the requisite accuracy and sensitivity to assay phytic acid incorn flour samples.
Keywords
, Phytic Acid Detection, Phytase Enzyme, Graphitic Carbon Nitride Nanosheets, Biosensor
@article{paperid:1078399,
author = {چکاوک اسماعیلی and پرویز نوروزی and Saiedeh Agh Atabay and Ahmadzadeh, Hossein and Kafshdare Goharshadi, Elaheh},
title = {Biosensing Applications of Graphitic Carbon Nitride-Chitosan Nanosheets on Glassy Carbon Electrode for Determination of Phytic Acid Biosensor},
journal = {Sensor Letters},
year = {2018},
volume = {16},
number = {8},
month = {August},
issn = {1546-198X},
pages = {573--579},
numpages = {6},
keywords = {Phytic Acid Detection; Phytase Enzyme; Graphitic Carbon Nitride Nanosheets;Biosensor},
}
%0 Journal Article
%T Biosensing Applications of Graphitic Carbon Nitride-Chitosan Nanosheets on Glassy Carbon Electrode for Determination of Phytic Acid Biosensor
%A چکاوک اسماعیلی
%A پرویز نوروزی
%A Saiedeh Agh Atabay
%A Ahmadzadeh, Hossein
%A Kafshdare Goharshadi, Elaheh
%J Sensor Letters
%@ 1546-198X
%D 2018
