Aims: Obesity and overweight are one of the main causes of some serious health problems such as hepatic disease. Among none-drugs approach for overweight management, physical exercise is in the first line. The purpose of this study was to determine the effect of eight weeks of high intensity interval training on the levels of enzymes involved in liver injury, lipid profile and body composition in overweight young men. Methods: In this study, 18 inactive 20 – 30 years old with a BMI of 25-30 kg/m2 were selected using convenience sampling and then randomly divided into two groups of HIIT (n= 10) and control group (N=8). The experimental group performed the exercise protocol three times a week for eight weeks. Blood samples were taken 24 h before the first and 48 h after the last training session to measure liver enzymes and lipid profile. Paired and independent t-tests were used to compare intra- and inter-group changes, respectively. Results: Based on the results of the present study, eight weeks of HIIT training significantly decreased AST, TG, BMI, PBF, WHR values (p<0/05) and a significant difference was observed in ALT, TG, AST and WHR values between the two groups (p<0/05). Conclusion: HIIT exercise could be an effective training method for improving liver enzymes and reducing body fat in obese young men.

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