Title : ( Data on analysis of OCC-1 transcript levels in pluripotent and differentiated states of P19 cells )
Authors: Zahra Hosseininia , Sara Soltanian , Nasser Mahdavi SHahri , Hesam Dehghani ,
Full TextAbstract
We investigated the expression of OCC-1 at mRNA level during retinoic acid (RA) induced differentiation of mouse embryonic carcinoma P19 pluripotent cancer cells by quantitative real time PCR (qPCR). By employing four-fold serial dilutions of P19 cDNA, standard curves were generated for the reference gene (L37) and the gene of interest (OCC-1). PCR efficiencies for L37 and OCC-1 were calculated. Since the amplification efficiencies of these two genes were unequal, the standard curve method was used for the relative quantification of OCC-1. Data analysis revealed that the expression of OCC-1 was reduced by about 69% after 4-day treatment with RA, when significant down-regulation of key pluripotency factors, including OCT4 and Nanog was observed [1].
Keywords
, OCC-1, P19, Pluripotency, Differentiation, Retinoic acid
@article{paperid:1079517,
author = {Hosseininia, Zahra and سارا سلطانیان and Mahdavi SHahri, Nasser and Dehghani, Hesam},
title = {Data on analysis of OCC-1 transcript levels in pluripotent and differentiated states of P19 cells},
journal = {Data in Brief},
year = {2020},
volume = {29},
number = {2},
month = {April},
issn = {2352-3409},
pages = {105367--105374},
numpages = {7},
keywords = {OCC-1; P19; Pluripotency; Differentiation; Retinoic acid},
}
%0 Journal Article
%T Data on analysis of OCC-1 transcript levels in pluripotent and differentiated states of P19 cells
%A Hosseininia, Zahra
%A سارا سلطانیان
%A Mahdavi SHahri, Nasser
%A Dehghani, Hesam
%J Data in Brief
%@ 2352-3409
%D 2020
