Background: Antimicrobial compounds have numerous applications, and essential oils could be used in edible films to enhance the food shelf life. This study aimed to assess the inhibitory effects of a chitosan/cellulose nanofiber (CNF) film containing the nanoemulsions of Bunium persicum essential oil (NBPEO) and Trachyspermum ammi essential oil (NTEO) on Escherichia coli O157:H7 inoculated in hamburger during storage (4°C). Methods: After inoculation, the hamburger samples were classified into three treatment groups of, control, chitosan containing 7.5% CNF (Ch-CNF), and chitosan containing 7.5% CNF enriched with 1.6% NTEO and 0.8% NBPEO (Ch-CNF-NEO). The samples were preserved at the temperature of 4°C, and the bacterial counts were determined on days zero, three, six, nine, and 12. Data analysis was performed using Bonferroni post-hoc test and repeated measures ANOVA. Results: The mean E.coli O157:H7 count significantly decreased in the treatments groups compared to the control group. In addition the Ch-CNF-NEO film exerted the most significant inhibitory effects on the growth of E. coli O157: H7 in the hamburger samples. Conclusion: According to the results, the Ch-CNF-NEO film could effectively reduce the growth of E. coli O157:H7 in hamburger. Therefore, Ch-CNF-NEO film could be used to effectively increase the safety of meat products against E. coli O157: H7.

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