Bioinspired, Biomimetic and Nanobiomaterials, Volume (10), No (1), Year (2021-3) , Pages (16-27)

Title : ( Induction of osteogenesis in bone tumour cells by purine-conjugated zinc-hydroxyapatite )

Authors: Azadeh Meshkini , elnaz sistanipour , Hamid Oveisi , Ahmad Asoodeh ,

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This study aimed to improve the biocompatibility and osteogenic property of hydroxyapatite (HAP). So HAP nanoparticles were doped with zinc (Zn), and their surface was modified with a purine nucleotide, guanosine 50-triphosphate (GTP). GTP-loaded nanoparticles (GTP@ZnHAP) were characterised by field emission scanning electron microscopy, Fourier transform infrared, thermogravimetric analysis, zeta potential and ultraviolet–visible spectroscopy. Biological experiments revealed that GTP@ZnHAP nanoparticles were internalised by the cells, inhibiting tumour cell (osteoblast-like cells, Saos-2) expansion with an efficiency more than that observed for ZnHAP nanoparticles and GTP alone. Furthermore, Saos-2 cells were committed to differentiate into the normal osteoblast cells under the influence of GTP@ZnHAP nanoparticles demonstrated by the quantitative assessment of bone-related protein expression (Runx2 and osteocalcin) and cell morphological changes. Moreover, high-performance liquid chromatography analyses disclosed a significant enhancement of intracellular GTP content in GTP@ZnHAP-treated cells, proposing perturbation of intracellular nucleotide equilibrium during the process of osteogenesis induced by GTP@ZnHAP nanoparticles. Overall, GTP@ZnHAP exhibits a better synergistic effect on the modulation of cell growth and induction of osteogenic differentiation in osteosarcoma cells than ZnHAP nanoparticles and GTP alone do. Therefore, GTP@ZnHAP may be regarded as a promising biomaterial for the treatment of bone-related diseases.


Apatite; Biomimetic materials; bone
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author = {Meshkini, Azadeh and Sistanipour, Elnaz and Hamid Oveisi and Asoodeh, Ahmad},
title = {Induction of osteogenesis in bone tumour cells by purine-conjugated zinc-hydroxyapatite},
journal = {Bioinspired, Biomimetic and Nanobiomaterials},
year = {2021},
volume = {10},
number = {1},
month = {March},
issn = {2045-9858},
pages = {16--27},
numpages = {11},
keywords = {Apatite; Biomimetic materials; bone},


%0 Journal Article
%T Induction of osteogenesis in bone tumour cells by purine-conjugated zinc-hydroxyapatite
%A Meshkini, Azadeh
%A Sistanipour, Elnaz
%A Hamid Oveisi
%A Asoodeh, Ahmad
%J Bioinspired, Biomimetic and Nanobiomaterials
%@ 2045-9858
%D 2021