Title : ( RNAi-mediated down-regulation of SHATTERPROOF gene in transgenic oilseed rape )
Authors: Hadis Kord , Ali Mohammad Shakib , Mohammad Hossein Daneshvar , Pejman Azadi , Vahid Bayat , Mohsen Mashayekhi , Mahboobeh Zare , Alireza Seifi , Mana Ahmad-Raji ,
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Abstract
Oilseed rape is one of the important oil plants. Pod shattering is one of the problems in oilseed rape production especially in regions with dry conditions. One of the important genes in Brassica pod opening is SHATTERPROOF1 (SHP1). Down-regulation of BnSHP1 expression by RNAi can increase resistance to pod shattering. A 470 bp of the BnSHP1 cDNA sequence constructed in an RNAi-silencing vector was transferred to oilseed rape cv. SLM046. Molecular analysis of T2 transgenic plants by RT-PCR and Real-time PCR showed that expression of the BnSHP alleles was highly decreased in comparison with control plants. Morphologically, transgenic plants were normal and produced seeds at greenhouse conditions. At ripening, stage pods failed to shatter, and a finger pressure was needed for pod opening.
Keywords
, BnSHP gene, Gene silencing, Oilseed rape, RNAi, Pod shattering
@article{paperid:1083649,
author = {Hadis Kord and Ali Mohammad Shakib and Mohammad Hossein Daneshvar and Pejman Azadi and Vahid Bayat and Mohsen Mashayekhi and Mahboobeh Zare and Seifi, Alireza and Mana Ahmad-Raji},
title = {RNAi-mediated down-regulation of SHATTERPROOF gene in transgenic oilseed rape},
journal = {3Biotech},
year = {2015},
volume = {5},
number = {3},
month = {June},
issn = {2190-572X},
pages = {271--277},
numpages = {6},
keywords = {BnSHP gene; Gene silencing; Oilseed rape;
RNAi; Pod shattering},
}
%0 Journal Article
%T RNAi-mediated down-regulation of SHATTERPROOF gene in transgenic oilseed rape
%A Hadis Kord
%A Ali Mohammad Shakib
%A Mohammad Hossein Daneshvar
%A Pejman Azadi
%A Vahid Bayat
%A Mohsen Mashayekhi
%A Mahboobeh Zare
%A Seifi, Alireza
%A Mana Ahmad-Raji
%J 3Biotech
%@ 2190-572X
%D 2015
