Current study was designed to evaluate the effects of b-cyfluthrin, as a toxicant substance, and trans-ferulic acid (trans-FA), as a protective agent, on different parameters of rooster semen upon liquid storage. For this purpose, semen samples of roosters (Ross 308, n = 10, 32-wk-old) were collected twice a week. Good quality samples (≥70% progressive motion) were diluted, pooled and then divided for the purposes of the study. In the first experiment, motility of spermatozoa was evaluated following exposure to different concentrations of b-cyfluthrin (1, 2.5, 5, 10, 20, 40, and 80 mM) at 0, 24, and 48 h of storage. In the second experiment, constant doses of b-cyfluthrin (10 mM) alone or in combination with trans-FA (10, 25 mM) were assessed on motility and viability of spermatozoa at 0, 24, and 48 h time points. Moreover, amounts of malondialdehyde (MDA), total antioxidant capacity (TAC), total nitrate-nitrite, total hydroperoxide (HPO), and activity of superoxide dismutase (SOD) were evaluated in the homogenate of spermatozoa-diluent at studied time points. Results of the first experiment showed that amounts of b-cyfluthrin greater than 5 mM, significantly reduced the motility of spermatozoa at 24 and 48 h of storage (P < 0.05). The second experiment demonstrated that, trans-FA especially at 10, 25 mM doses restored the motility and viability of spermatozoa compared to b-cyfluthrin treated group (P < 0.05). Amounts of MDA (10, 25 mM), hydroperoxide (10, 25, and 50 mM), and nitrate-nitrite (10, 25, and 50 mM) were lower and TAC (10 and 25 mM) were greater in trans-FA + bcyfluthrin treated groups compared to b-cyfluthrin alone treated samples (P < 0.05). However, activity of SOD did not show significant changes by the treatment (P > 0.05). It seems that trans-FA could ameliorate toxic effect of b-cyfluthrin via reduction of peroxidative (as evident by measurement of MDA) and nitrosative (as evident by measurement of nitrate-nitrite) reactions over cold preservation of rooster semen.

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