Title : ( Evaluation of synergistic effects of cisplatin and methotrexate on proliferation and death of colorectal cancer cells )
Authors: Azadeh Meshkini , laya khoshtabiat , Maryam Moghaddam Matin ,
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Abstract
Cisplatin (CDDP) is one of the most frequently used chemotherapy drugs, displays a strong therapeutic effect against cancer. Predominantly, CDDP binds to the DNA, prevents transcription and replication, and induces programmed cell death. Nevertheless, some types of cancer, like colorectal cancer, are very resistant to cisplatin. It leads to a large number of side effects and tumor relapse in treated patients. To overcome these problems, combination chemotherapy is a superior treatment strategy. The present study aimed to evaluate the therapeutic efficacy and safety of the combination of CDDP and methotrexate (MTX) against colon cancer. So, the effect of cytotoxicity and induction of apoptosis of a single drug (CDDP or MTX) or combination of CDDP plus MTX on CT26 colorectal cancer were studied, by MTT assay and Ao/EtBr staining. The results were revealed an intense synergistically cytotoxic effect in CT26 colorectal cancer when they were co-treated with CDDP andMTX. Also, staining of samples with Ao/EtBr showed an increase in induction of apoptosis, as programmed death, in CDDP and MTX-treated cells, compared with that in single drug-treated cells
Keywords
, Cancer, Combination Therapy, Cisplatin, Methotroxate, CT26 Cells
@inproceedings{paperid:1086195,
author = {Meshkini, Azadeh and Khoshtabiat, Laya and Moghaddam Matin, Maryam},
title = {Evaluation of synergistic effects of cisplatin and methotrexate on proliferation and death of colorectal cancer cells},
booktitle = {9th international congress on Biology},
year = {2021},
location = {سمنان, IRAN},
keywords = {Cancer; Combination Therapy; Cisplatin; Methotroxate; CT26 Cells},
}
%0 Conference Proceedings
%T Evaluation of synergistic effects of cisplatin and methotrexate on proliferation and death of colorectal cancer cells
%A Meshkini, Azadeh
%A Khoshtabiat, Laya
%A Moghaddam Matin, Maryam
%J 9th international congress on Biology
%D 2021
