Title : ( Decellularized bovine aorta as a promising 3D elastin scaffold for vascular tissue engineering applications )
Authors: Tahmineh Kazemi , Ahmad Ali Mohammadpour , Maryam Moghaddam Matin , Nasser Mahdavi SHahri , Hesam Dehghani , Seyed Hasan Kazemi Riabi ,
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Abstract
Aim: To evaluate the suitability of using aorta elastin scaffold, in combination with human adipose-derived mesenchymal stem cells (hAd-MSCs), as an approach for cardiovascular tissue engineering. Materials & Methods: Human adipose-derived MSCs were seeded on elastin samples of decellularized bovine aorta. The samples were cultured in vitro to investigate the inductive effects of this scaffold on the cells. The results were evaluated using histological, and immunohistochemical methods, as well as MTT assay, DNA content, reverse transcription-PCR and scanning electron microscopy. Results: Histological staining and DNA content confirmed the efficacy of decellularization procedure (82% DNA removal). MTT assay showed the construct’s ability to support cell viability and proliferation. Cell differentiation was confirmed by reverse transcription-PCR and positive immunohistochemistry for alfa smooth muscle actin and von Willebrand. Conclusion: The prepared aortic elastin samples act as a potential scaffold, in combination with MSCs, for applications in cardiovascular tissue engineering. Further experiments in animal models are required to confirm this.
Keywords
aorta regeneration • cardiovascular tissue engineering • elastin scaffold • extracellular matrix • mesenchymal stem cells • regenerative medicine
@article{paperid:1087837,
author = {Kazemi, Tahmineh and Mohammadpour, Ahmad Ali and Moghaddam Matin, Maryam and Mahdavi SHahri, Nasser and Dehghani, Hesam and Kazemi Riabi, Seyed Hasan},
title = {Decellularized bovine aorta as a promising 3D elastin scaffold for vascular tissue engineering applications},
journal = {Regenerative Medicine},
year = {2021},
volume = {16},
number = {12},
month = {December},
issn = {1746-0751},
pages = {1037--1050},
numpages = {13},
keywords = {aorta regeneration • cardiovascular tissue engineering • elastin scaffold • extracellular matrix
• mesenchymal stem cells • regenerative medicine},
}
%0 Journal Article
%T Decellularized bovine aorta as a promising 3D elastin scaffold for vascular tissue engineering applications
%A Kazemi, Tahmineh
%A Mohammadpour, Ahmad Ali
%A Moghaddam Matin, Maryam
%A Mahdavi SHahri, Nasser
%A Dehghani, Hesam
%A Kazemi Riabi, Seyed Hasan
%J Regenerative Medicine
%@ 1746-0751
%D 2021
