Molecular Genetics and Genomics, Volume (297), No (4), Year (2022-6) , Pages (1-9)

Title : ( MS-HRM protocol: a simple and low-cost approach for technical validation of next-generation methylation sequencing data )

Authors: Ali Javadmanesh , Afsaneh Mojtabanezhad Shariatpanahi , Ehsan Shams Davodly , marjan azghandi , Maryam Yassi , Mehdi Heidari , Matin Kerachian , Mohammad Amin Kerachian ,

Access to full-text not allowed by authors

Citation: BibTeX | EndNote

Abstract

DNA methylation is a fundamental epigenetic process and have a critical role in many biological processes. The study of DNA methylation at a large scale of genomic levels is widely conducted by several techniques that are next-generation sequencing (NGS)-based methods. Methylome data revealed by DNA methylation next-generation sequencing (mNGS), should be always verified by another technique which they usually have a high cost. In this study, we offered a low-cost approach to corroborate the mNGS data. In this regard, mNGS was performed on 6 colorectal cancer (case group) and 6 healthy individual colon tissue (control group) samples. An R-script detected differentially methylated regions (DMRs), was further validated by high resolution melting (MS-HRM) analysis. After analyzing the data, the algorithm found 194 DMRs. Two locations with the highest level of methylation difference were verified by MS-HRM, which their results were in accordance with the mNGS. Therefore, in the present study, we suggested MS-HRM as a simple, accurate and low-cost method, useful for confirming methylation sequencing results.

Keywords

Bisulfite; DNA Methylation Sequencing; High resolution melting; Validation
برای دانلود از شناسه و رمز عبور پرتال پویا استفاده کنید.

@article{paperid:1090207,
author = {Javadmanesh, Ali and افسانه مجتبی نژاد شریعت پناهی and احسان شمس داودلی and Azghandi, Marjan and مریم یاسی and مهدی حیدری and متین کراچیان and محمد امین کراچیان},
title = {MS-HRM protocol: a simple and low-cost approach for technical validation of next-generation methylation sequencing data},
journal = {Molecular Genetics and Genomics},
year = {2022},
volume = {297},
number = {4},
month = {June},
issn = {1617-4615},
pages = {1--9},
numpages = {8},
keywords = {Bisulfite; DNA Methylation Sequencing; High resolution melting; Validation},
}

[Download]

%0 Journal Article
%T MS-HRM protocol: a simple and low-cost approach for technical validation of next-generation methylation sequencing data
%A Javadmanesh, Ali
%A افسانه مجتبی نژاد شریعت پناهی
%A احسان شمس داودلی
%A Azghandi, Marjan
%A مریم یاسی
%A مهدی حیدری
%A متین کراچیان
%A محمد امین کراچیان
%J Molecular Genetics and Genomics
%@ 1617-4615
%D 2022

[Download]