Biomass Conversion and Biorefinery, Year (2022-12)

Title : ( Sugar beet molasses bioconversion into biosurfactant: optimization and comparison with other carbon sources )

Authors: mahdi shahabi , Akram Halajnia , Amir Lakzian , Mohammad Reza Housaindokht ,

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Abstract

Interest in biosurfactants application has increased considerably due to unique properties, such as diversity, biodegradability and environmentally friendly concerns. Therefore, using abundant and low-cost raw materials and optimizing the biosurfactant production process are economically attractive. In this study, the suitability of sugar beet molasses was compared with some other common carbon sources for rhamnolipids production by Pseudomonas putida KT2440. The effects of different inorganic nitrogen sources, C/N ratios, and incubation time were also investigated to optimize biosurfactant production. The results showed that the maximum biosurfactant precipitated in acetone (5.4 g.L) was obtained using molasses and sodium nitrate. In the application of these carbon and nitrogen sources, minimum surface tension was established at C/N ratio of 10 and 8 days of incubation time. Afected by various sources of carbon and nitrogen, the inal pH values varied between 2.9 and 8.3. Although the minimum surface tension (41.78 m.Nm-1) was measured using olive oil and ammonium chloride, this treatment did not produce the maximum biosurfactant weight. The minimum pH of cell-free supernatants was related to this treatment. In other words, the inal pH of the culture medium was efective in measuring parameters related to biosurfactant. Acetone-extracted biosurfactant was pH-dependent and increased with the increasing pH of the culture mediums. The results indicated the potential of sugar beet molasses as a hydrophilic agro-industrial wastes to rhamnolipid production by Pseudomonas putida KT2440.

Keywords

Rhamnolipid · Hydrophilic substrate · Molasses · Optimization · pH
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@article{paperid:1092574,
author = {Shahabi, Mahdi and Halajnia, Akram and Lakzian, Amir and Housaindokht, Mohammad Reza},
title = {Sugar beet molasses bioconversion into biosurfactant: optimization and comparison with other carbon sources},
journal = {Biomass Conversion and Biorefinery},
year = {2022},
month = {December},
issn = {2190-6815},
keywords = {Rhamnolipid · Hydrophilic substrate · Molasses · Optimization · pH},
}

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%0 Journal Article
%T Sugar beet molasses bioconversion into biosurfactant: optimization and comparison with other carbon sources
%A Shahabi, Mahdi
%A Halajnia, Akram
%A Lakzian, Amir
%A Housaindokht, Mohammad Reza
%J Biomass Conversion and Biorefinery
%@ 2190-6815
%D 2022

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