International Congress on Domestic Animal Breeding, Genetics and Husbandry - 2022 (ICABGEH-22) , 2022-10-03

Title : ( INSERTION AND ACTIVATION OF A REPORTER INTO THE 3’-UTR REGION OF THE OVALBUMIN GENE IN CHICKEN FIBROBLASTS USING CRISPR/DCAS9-MEDIATED TRANSPOSITION )

Authors: lena goshayeshi , nima dehdilani , Sara Yousefi Taemeh , Hesam Dehghani ,

Citation: BibTeX | EndNote

Abstract

CRISPR technology is the most robust genome manipulation tool that allows researchers to generate animals with new features. Using CRISPR technology various animals with an improved immune system, disease resistance, and the ability in producing drugs have been generated. In transgenic chicken, under the control of the ovalbumin gene promoter, biopharmaceutical proteins are produced in the oviduct and secreted into the egg white. In this study to exploit the promoter of the ovalbumin gene, we inserted a promoterless reporter gene in the 3’-UTR region of this gene in chicken DF1 cells using CRISPR-HDR. Then, we applied our recently developed CRISPR/dCas9-mediated transposition technology to incorporate a ubiquitous promoter (CMV), upstream of ovalbumin exon 1 and to activate this promoter in DF1 cells. Our results showed that the 3’UTR region of the ovalbumin gene is a suitable candidate to support the expression of the transgene in chicken bioreactors. Also, our introduced dCas9-piggyBac transposition system provided a useful tool for studying transgene expression in non-oviduct cells. These methods provide useful tools for the generation of transgenic chicken bioreactors.

Keywords

, CRISPR/Cas9 system, Biopharmaceutical proteins, CRISPR/dCas9-mediated transposition, Oviduct-specific expression
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@inproceedings{paperid:1094074,
author = {Goshayeshi, Lena and Dehdilani, Nima and Yousefi Taemeh, Sara and Dehghani, Hesam},
title = {INSERTION AND ACTIVATION OF A REPORTER INTO THE 3’-UTR REGION OF THE OVALBUMIN GENE IN CHICKEN FIBROBLASTS USING CRISPR/DCAS9-MEDIATED TRANSPOSITION},
booktitle = {International Congress on Domestic Animal Breeding, Genetics and Husbandry - 2022 (ICABGEH-22)},
year = {2022},
location = {سامسون, IRAN},
keywords = {CRISPR/Cas9 system; Biopharmaceutical proteins; CRISPR/dCas9-mediated transposition; Oviduct-specific expression},
}

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%0 Conference Proceedings
%T INSERTION AND ACTIVATION OF A REPORTER INTO THE 3’-UTR REGION OF THE OVALBUMIN GENE IN CHICKEN FIBROBLASTS USING CRISPR/DCAS9-MEDIATED TRANSPOSITION
%A Goshayeshi, Lena
%A Dehdilani, Nima
%A Yousefi Taemeh, Sara
%A Dehghani, Hesam
%J International Congress on Domestic Animal Breeding, Genetics and Husbandry - 2022 (ICABGEH-22)
%D 2022

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