Here, we report the effects of a single abscisic acid (ABA) spray on Arabidopsis seedlings on growth, development, primary metabolism, and response to water-deficit stress in adult and next-generation plants. The experiments were performed over 2 years in two different laboratories in Iran and South Africa. In each experiment, fifty 7-day-old Arabidopsis seedlings were sprayed with 10 μM ABA, 1 mM H2O2, distilled water, or left without spraying as priming treatments. Water-deficit stress was applied on half of the plants in each treatment by withholding water 2 days after spraying. Results showed that a single ABA spray at the cotyledonary stage significantly increased plant biomass and delayed flowering. The ABA spray significantly enhanced drought tolerance so that the survival rate after rehydration was 100 and 33% in the first and the second experiments, respectively, for ABA-treated plants compared to 35 and 0% for water-sprayed plants. This enhanced drought tolerance was not inheritable. Metabolomics analyses suggested that ABA probably increases the antioxidant capacity of the plant cells and modulates tricarboxylic acid cycle toward enhanced nitrogen assimilation. Strikingly, we also observed that the early water spray decreases mature plant resilience under water-deficit conditions and cause substantial transient metabolomics perturbations.

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