Small Ruminant Research, ( ISI ), Volume (113), No (1), Year (2013-6) , Pages (297-300)

Title : ( Prevalence of Shiga toxin-producing and enteropathogenic Escherichia coli in slaughtered camels in Iran )

Authors: Tabatabaei , Taghi Zahraei Salehi , Mahdi Askari Badouei , Ashrafi Tamai , Akbarinejad , Kazempoor , M. Shojaei ,

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Abstract

To investigate the prevalence of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains in Iranian camels, E. coli strains isolated from fecal samples of 125 slaughtered camels were subjected to PCR for detection of stx1, stx2, eaeA and Ehly genes. The strains determined positive were then tested for O157:H7, O111 and O26 serotypes and bfpA gene using PCR. In addition, the intimin subtype of eaeA-positive isolates was determined by PCR-RFLP and sequencing. EPEC strains, none of which belonged to the tested serogroups, were detected in 2.4% (3 out of 125) of stool samples, whereas no STEC strain was detected. The eaeA-positive isolates were considered as atypical EPEC due to lack of bfpA gene, two of which contained beta intimin subtype. To our knowledge, this is the first molecular description of EPEC strains in camels.

Keywords

, Prevalence Shiga toxin, producing E. coli Enteropathogenic E. coli Camel PCR
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@article{paperid:1095714,
author = {Tabatabaei and Taghi Zahraei Salehi and Askari Badouei, Mahdi and Ashrafi Tamai and Akbarinejad and Kazempoor and M. Shojaei},
title = {Prevalence of Shiga toxin-producing and enteropathogenic Escherichia coli in slaughtered camels in Iran},
journal = {Small Ruminant Research},
year = {2013},
volume = {113},
number = {1},
month = {June},
issn = {0921-4488},
pages = {297--300},
numpages = {3},
keywords = {Prevalence Shiga toxin-producing E. coli Enteropathogenic E. coli Camel PCR},
}

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%0 Journal Article
%T Prevalence of Shiga toxin-producing and enteropathogenic Escherichia coli in slaughtered camels in Iran
%A Tabatabaei
%A Taghi Zahraei Salehi
%A Askari Badouei, Mahdi
%A Ashrafi Tamai
%A Akbarinejad
%A Kazempoor
%A M. Shojaei
%J Small Ruminant Research
%@ 0921-4488
%D 2013

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