Background: In recent years, there is a growing interest in the creation of biocompatible nanostructures for tissue engineering, which are classified into polymer, carbon, mineral, and composite kinds based on the component materials and manufacture techniques. Materials and Methods: In this study, we tried to optimize the method of creating a biocompatible hybrid nanostructure composed of type 1 collagen and polyvinyl alcohol polymer (PVA) with an alcoholic extract of propolis. Polyvinyl alcohol is a biocompatible and non-conductive polymer; Type 1 collagen is extracted from mouse tails as a natural ECM material, and bee propolis is known for its natural anti-inflammatory and antimicrobial properties. The nanostructure was created using an electrospinning machine. Based on the assessments during the nanostructure creation process, it was determined that the most effective parameter in collagen electrospinning is solvent type, solvent concentration and feeding rate. Results: Here, phosphate-buffered saline (PBS) and 70% ethanol were used as collagen solvents, while PVA was dissolved in deionized water. For propolis, its flavonoids were measured and a suitable method without heating was selected for extraction. At the end, the physical properties of the produced nanostructures were examined using SEM and spectrometry. The results showed that the diameter of hybrid nanofibers is affected by the type and concentration of the solvent, as well as the solution\\\'s viscosity. Conclusion: In conclusion, setting the optimized parameters result in the formation of the best matrix with small fibers and high porosity for tissue engineering purposes.

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