Background: The selection of oocytes in assisted reproductive technology (ART) primarily relies on morphological characteristics, however, the current grading and screening criteria still contain subjective elements. Here, we assessed the morphometric (instead of morphological) characteristics of oocytes which were selected as the most competent candidates for intracytoplasmic sperm injection (ICSI) procedure. Materials and Methods: Here, we assessed the morphometric (instead of morphological) characteristics of oocytes which were selected as the most competent candidates for intracytoplasmic sperm injection (ICSI) procedure. 30 women, mean aged of 31.9 years, underwent controlled ovarian stimulation for ICSI. A total of 115 oocytes were collected. The oocyte samples were categorized as low and high quality by an embryologist and subjected to imaging. Then, various morphometric characteristics were analyzed using ImageJ software (Version 1.54b). The analysis was included measurements such as oocyte diameter and area, ooplasm area and diameter, the range of perivitelline space width (minimum and maximum), zona pellucida thickness, dimensions (diameter and area) of the first polar body, as well as the ratio between the ooplasm area and polar body area. Results: The results showed that the mean diameters and the area of the ooplasm in competent oocytes, were 116.3 μm and 11.1*104 μm2, respectively, which were not significantly different from those of low-quality oocytes (112.4 μm and 9.7*104 μm2). The maximum width of the perivitelline space in competent oocytes was equal to the diameter of the polar body and its location. The ratio of the area of the ooplasm to the polar body area of the competent oocyte was 31.5 ± 5.2, and the quality of the prepared zygotes was better in this range. The average thickness of the zona pellucida was 17.6 μm, with competent oocytes displayed a uniform and thinner zona pellucida compared to low-quality oocytes. Conclusion: It can be concluded that morphometric characteristics is more accurate than morphological characteristics for oocyte selection in ART centers which can increase the rate of pregnancy.

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