This study developed a nanoantioxidant-based active food packaging film with antibacterial activity, low cytotoxicity, and potential for food preservation. Ag-doped ZnO nanoparticles (ZnO.Ag NPs) were synthesized with antioxidant and UV-shielding properties and loaded with catechin (Cat) to enhance biological activity. These nanoantioxidants were incorporated into chitosan/poly(vinyl alcohol) (CS/PVA) films (CP.(ZAC)) at varying concentrations. Adding ZnO.Ag@Cat reduced the water contact angle (89° to 78°) and water vapor permeability (121 × 10-4 to 99 × 10-4 g.Pa-1.day-1.m-1). The films exhibited nearly 100% antibacterial efficacy against Staphylococcus aureus and Escherichia coli. Molecular docking revealed that catechin inhibited bacterial virulence factors, including SarA and CRP proteins. CP.(ZAC)9 extended strawberry shelf life at 4 °C to 12 days while maintaining quality indices such as weight loss, total soluble solids (TSS), pH, and electrical resistance. The antioxidant properties of CP.(ZAC)9 mitigated oxidative stress, reducing H2O2 accumulation in strawberries. These findings demonstrate the potential of nanoantioxidant-based films to enhance shelf life and quality, offering a sustainable alternative for food preservation.

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