Objective: Tissue engineering aims to repair and restore the function of damaged tissues using cells, scaffolds, and bioac- tive molecules. Biopolymeric materials are of significant interest in scaffold fabrication due to their inherent biocompatibility and therapeutic properties. Among various fiber production techniques, electrospinning uniquely enables the fabrication of scaffolds with structures closely resembling the extracellu- lar matrix (ECM), producing fibers ranging from the nano- to microscale. In this study, biocompatible fibers composed of polyvinyl alcohol (PVA) and Origanum vulgare extract were fabricated via electrospinning and evaluated for biomedical ap- plications. Water-soluble PVA was used as the scaffold base, while Origanum vulgare extract—rich in anti-inflammatory and antioxidant bioactive compounds—was incorporated to en- hance both structural support and tissue healing. Materials and Methods: A 10 wt% PVA solution was prepared by heating to 80°C and then cooled to room temperature. Ori- ganum vulgare extract was obtained through hydroalcoholic extraction and rotary evaporation, and added to the polymer so- lution at a defined weight ratio. Electrospinning was performed under standardized conditions: 20 kV applied voltage, ambi- ent temperature of 23°C, and relative humidity of 47%. Fiber morphology was analyzed using SEM, while FTIR was used to evaluate interactions between functional groups. Cytotoxicity was assessed via MTT assay using human HDF cells seeded at a density of 5000 cells per well in 96-well plates, with exposure durations of 24 and 48 hours. Results: SEM imaging revealed uniform, bead-free fibers with average diameters of 27, 30, and 36 μm. FTIR spectra dis- played sharp peaks in the wavenumber ranges of approximately 2900 and 3286 cm⁻¹, indicating interactions between hydroxyl groups in PVA and phenolic compounds in Origanum vulgare. MTT assay results showed no cytotoxic effects on HDF cells at either time point. Conclusion: Electrospun PVA scaffolds incorporating Origa- num vulgare extract exhibited high biocompatibility and poros- ity. In addition to offering protection against oxidative stress and inflammation, the scaffolds also promoted the proliferation of skin fibroblasts. These findings suggest potential for future applications in wound healing and tissue regeneration studies.

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