Cell Stem Cell, ( ISI ), Year (2008-5) , Pages (437-447)

Title : Promiscuous global transcription in pluripotent embryonic stem cells ( Promiscuous global transcription in pluripotent embryonic stem cells )

Authors: Efroni S , Duttagupta R , Cheng J , Hesam Dehghani , Hoeppner DJ , Dash C , Bazett-Jones D , Le Grice S , Mckay RDG , Buetow KH , Gingeras T , Misteli T , Meshorer E ,

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Abstract

The molecular mechanisms underlying pluripotency and lineage specification from embryonic stem cells (ESCs) are largely unclear. Differentiation pathways may be determined by the targeted activation of lineage-specific genes or by selective silencing of genome regions. Here we show that the ESC genome is transcriptionally globally hyperactive and undergoes large-scale silencing as cells differentiate. Normally silent repeat regions are active in ESCs, and tissuespecific genes are sporadically expressed at low levels.Whole-genometilingarraysdemonstrate widespread transcription in coding and noncoding regions in ESCs, whereas the transcriptional landscape becomes more discrete as differentiation proceeds. The transcriptional hyperactivity in ESCs is accompanied by disproportionate expression of chromatin-remodeling genes andthegeneral transcriptionmachinery. Wepropose that global transcription is a hallmark of pluripotent ESCs, contributing to their plasticity, and that lineage specification is driven by reduction of the transcribed portion of the genome.

Keywords

, stem Cell, pluripotency, transcription
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@article{paperid:204535,
author = {Efroni S and Duttagupta R and Cheng J and Dehghani, Hesam and Hoeppner DJ and Dash C and Bazett-Jones D and Le Grice S and Mckay RDG and Buetow KH and Gingeras T and Misteli T and Meshorer E},
title = {Promiscuous global transcription in pluripotent embryonic stem cells},
journal = {Cell Stem Cell},
year = {2008},
month = {May},
issn = {1934-5909},
pages = {437--447},
numpages = {10},
keywords = {stem Cell; pluripotency; transcription},
}

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%0 Journal Article
%T Promiscuous global transcription in pluripotent embryonic stem cells
%A Efroni S
%A Duttagupta R
%A Cheng J
%A Dehghani, Hesam
%A Hoeppner DJ
%A Dash C
%A Bazett-Jones D
%A Le Grice S
%A Mckay RDG
%A Buetow KH
%A Gingeras T
%A Misteli T
%A Meshorer E
%J Cell Stem Cell
%@ 1934-5909
%D 2008

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