Despite the establishment of extensive and successful control programmes in some countries or regions, Echinococcus granulosus (E. granulosus ) still has a very wide geographic distribution. One hundred and twenty of mature E. granulosus obtained from parasitology Depatrment, School of Veterniary Medicine, Ferdowsi University of Mashhad. Protein souble of whole body of parasite was prepared by feeeze-thawing in liqiued nitrogen and 42º C for three times. The sample hemogenized in a blender, sonicated in a 110 V, 170 W for 3x15 sec, contrifugated for 15min at 10000 g respectively. Final yield was kept in -20 º C. Ten mice were randomly divided into 2 groups of 5 mice. Each mouse in test group was vaccinated subcoutanusly with 100 μg (100 μl) of whole body of E. granulosus plus 100 μl of Freund ,s complete adjuvant (FCA) respectively. Mice in control group were vaccinated with adjuvant in PBS. Second vaccination was conducted after four weeks with the same preparation except that FCA was replaced by Freund ,s incomplete adjuvant (FIA). Three weeks after the second vaccination, each mouse was challenged with 2000 protoscolices intrapritoanally. Autopsies of mice were carried out 8 months post challenge. For the first time, our results were shown that in none of the vaccinated mice with the whole body of E. granulosus, cysts were not observed. This result indicated that the protective immunity in vaccinated mice was 100%. Key words: Hydatidosis, Echinococcus granulosus, whole body, mouse

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