Asian-Australasian Journal of Animal Sciences, ( ISI ), Volume (21), No (4), Year (2008-1) , Pages (465-470)

Title : The diversity of BOLA- DRB3 gene in Iranian native cattle ( The diversity of BOLA- DRB3 gene in Iranian native cattle )

Authors: Mohammadreza Nassiri , Feradoun EFTEKHARI SHAHROUDI , Mojtaba Tahmoorespur , Ali Javadmanesh ,

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This study describes genetic variability in the BoLA-DRB3 gene in Iranian native cattle (Bos Indicus and Taurus) and relationships between these breeds. This is the first study of genetic polymorphism of the BoLA-DRB3 gene in Iranian native cattle. We examined exon 2 of the major histocompatibility complex (MHC) class II DRB3 gene from 203 individuals in four populations of Iranian native cattle (52 Sarabi, 52 Najdi, 49 Sistani, 50 Golpayegani cattle) using the hemi-nested PCR-RFLP method. We identified the 36 previously reported alleles and one novel pattern (*eac). Analysis of the frequencies of the various BoLA-DRB3.2 alleles in each breed indicated that DRB3.2*52 in Sarabi cattle (23%), DRB3.2 *14 and *24 alleles in Najdi cattle (13%), DRB3.2 *8 allele in Sistani cattle (22%) and DRB3.2*16 allele in Golpayegani cattle (14%), were the most frequent alleles. Allelic frequencies ranged from 1 to 23% among the 36 alleles and there were some alleles that were found only in Iranian cattle. Effective number of alleles in the four breeds was estimated to be 7.86, 11.68, 7.08 and 3.37 in Sarabi, Najdi, Sistani and Golpayegani, respectively. Observed heterozygosities were the highest in Sarabi (94%) and Najdi (94%). A population tree based on the frequency of BoLA-DRB3.2 alleles in each breed suggested that Najdi, Sarabi and Golpayegani cattle clustered together and Najdi and Sarabi were the closest breeds. Sistani cattle differed more from these three breeds. These new data suggest that allele frequencies differ between Iranian cattle breeds.


, Key Words : BoLA-DRB3, PCR-RFLP, Iranian Native Cattle, MHC
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author = {Nassiri, Mohammadreza and EFTEKHARI SHAHROUDI, Feradoun and Tahmoorespur, Mojtaba and Javadmanesh, Ali},
title = {The diversity of BOLA- DRB3 gene in Iranian native cattle},
journal = {Asian-Australasian Journal of Animal Sciences},
year = {2008},
volume = {21},
number = {4},
month = {January},
issn = {1011-2367},
pages = {465--470},
numpages = {5},
keywords = {Key Words : BoLA-DRB3; PCR-RFLP; Iranian Native Cattle; MHC},


%0 Journal Article
%T The diversity of BOLA- DRB3 gene in Iranian native cattle
%A Nassiri, Mohammadreza
%A Tahmoorespur, Mojtaba
%A Javadmanesh, Ali
%J Asian-Australasian Journal of Animal Sciences
%@ 1011-2367
%D 2008