In an experiment the emotional and exploratory of Wistar rats to dietary zinc supplementation from zinc sulfate monohydrate (ZnS) , Zinc Oxide (ZnO) and Zinc Methionin (ZnM) were studied in an open field test .One hundred –twenty rats were assigned to 10 dietary treatments including : basal Diet (BD)+50 mg kg-1 ZnS, BD+100 mg kg-1 ZnS, Bd+200mg kg-1 ZnS, BD+50 mf kg-1 ZnO, BD+100 mg kg-1 ZnO, Bd+200mg kg-1 ZnO, BD+50 mf kg-1 ZnM, BD+100 mg kg-1 ZnM, Bd+200mg kg-1 ZnM, and basal diet (without Zn supplementation). These parameters including rearing, number of peripheral squares crossing, number of central squares crossing, hole exploration and defecation were recorded for 5 min. Treatments differed significantly in case of hole exploration and defecation. The results of these experiments indicated that high level of zinc methionin (200 mg kg-1) increased exploratory activity and locomotors activity. The results of this experiment indicated that high level of zinc methionin (200 mg kg-1) reduced exploratory activity and anxiety-like behavior. Further studies are needed to identify the anxiolytic mechanism and the optimum level of Zn supplementation

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