Molecular Breeding, ( ISI ), No (24), Year (2009-7) , Pages (17-24)

Title : ( Comparison of CEL I gene expression and mismatch-cleavage activity in some Apiaceae plants )

Authors: , Mohammad Farsi , Ahmad Reza Bahrami , Maryam Moghaddam Matin , وحید رضا یسائی ,

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The most familiar enzyme in mutation screening through heteroduplex analysis, CEL I, has been isolated from celery of the Apiaceae family. In this study, in a search for new sources with the same or better enzymatic activity, we studied the mismatch-cleavage activity of plant juice extracts from several Apiaceae plants (celery, carrot, coriander, parsley, dill, and fennel). This study was then followed by investigation of the level of CEL I gene expression in these plants. Mismatch-cleavage activity of fennel and dill juice extracts was lower than that of celery juice extract, and levels of CEL I mRNA expression in these plants were substantially higher than in celery. In contrast, the ability of juice extract from a local cultivar of parsley to cleave heteroduplex DNA substrates was clearly more than that of celery juice extract, whereas the level of CEL I gene expression in parsley was obviously lower than in celery. We concluded that there are multiple mismatch-cleaving enzymes collaborating in digestion of heteroduplex DNA substrates by plant juice extracts.

Keywords

, CEL I enzyme , Semi, quantitative RT, PCR , Western blotting , Heteroduplex analysis ,
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@article{paperid:1011117,
author = {, and Farsi, Mohammad and Bahrami, Ahmad Reza and Moghaddam Matin, Maryam and وحید رضا یسائی},
title = {Comparison of CEL I gene expression and mismatch-cleavage activity in some Apiaceae plants},
journal = {Molecular Breeding},
year = {2009},
number = {24},
month = {July},
issn = {1380-3743},
pages = {17--24},
numpages = {7},
keywords = {CEL I enzyme - Semi-quantitative RT-PCR - Western blotting - Heteroduplex analysis - Apiaceae},
}

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%0 Journal Article
%T Comparison of CEL I gene expression and mismatch-cleavage activity in some Apiaceae plants
%A ,
%A Farsi, Mohammad
%A Bahrami, Ahmad Reza
%A Moghaddam Matin, Maryam
%A وحید رضا یسائی
%J Molecular Breeding
%@ 1380-3743
%D 2009

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