Title : ( Use of real-time polymerase chain reaction assay for monitoring in vitro ruminal cellulolytic bacteria population as affected by non-structural carbohydrates )
Authors: Mohsen Danesh Mesgaran , , Alireza Heravi Moussavi , Seyed Alireza Vakili , Mohammadreza Nassiri , Shahrokh Ghovvati Roudsari ,
Full TextAbstract
Cellulose is the most abundant polymer in nature, but mammals do not synthesize enzymes to digest cellulose. Mosoni et al (2007) demonstrated that the dynamics of cellulolytic bacteria were in good correlation with the response to diet shift, particularly the changes of concentrate. The aim of the present study was to determine the effect of adding of non-structural carbohydrates (NSC) including sucrose, starch and equal mixture of them to an in vitro medium containing cellulose (Cell) as sole nutrient on both ruminal total anaerobic bacteria population and two major cellulolytic bacterial species (Fibrobacter succinogenes and Ruminococcus Albus) using SYBR Green real-time polymerase chain reaction (PCR) assay.
Keywords
, real-time polymerase chain reaction, in vitro ruminal cellulolytic bacteria, non-structural carbohydrates
@inproceedings{paperid:1015967,
author = {Danesh Mesgaran, Mohsen and , and Heravi Moussavi, Alireza and Vakili, Seyed Alireza and Nassiri, Mohammadreza and Ghovvati Roudsari, Shahrokh},
title = {Use of real-time polymerase chain reaction assay for monitoring in vitro ruminal cellulolytic bacteria population as affected by non-structural carbohydrates},
booktitle = {British Society of Animal Science},
year = {2010},
location = {ENGLAND},
keywords = {real-time polymerase chain reaction; in vitro ruminal cellulolytic bacteria; non-structural carbohydrates},
}
%0 Conference Proceedings
%T Use of real-time polymerase chain reaction assay for monitoring in vitro ruminal cellulolytic bacteria population as affected by non-structural carbohydrates
%A Danesh Mesgaran, Mohsen
%A ,
%A Heravi Moussavi, Alireza
%A Vakili, Seyed Alireza
%A Nassiri, Mohammadreza
%A Ghovvati Roudsari, Shahrokh
%J British Society of Animal Science
%D 2010
