This experiment was conducted to determine the effect of different sources of pectin feedstuffs on blood metabolites in early lactating Holstein cows. Eight primiparous early lactating Holstein cows (60 ± 23 days postpartum, weighing 530 ± 60 kg) were assigned into a duplicated 4 × 4 Latin square design with four 3-wk periods. Cows were allocated into four diets whit 1)10% barely grain, 2)10% sugar beet pulp, 3)10% wheat bran and 4) 10% dried citrus pulp . Each experimental period was 21 days including 14 days adaptation period and 7 days collecting samples. Blood samples were collected from the jugular vein 2h after the morning feeding. Blood serum was collected after centrifuged at 1,500 x g for 20 min, frozen at – 40°C, and later analyzed for Glucose, urea N, Cholesterol, Triglyceride, Alkaline phosphate and Albumin. There were no differences (p<0.05) on daily intake of DM (kg/d). Similarly, there were no significantly differences between treatments for blood metabolites. These data suggest that, the addition of sources of pectin feedstuffs at 10% levels (dry matter basis) of the dairy cow ration instead of cereal grain; will decrease the cost of milk production without any negative effect on DMI and Blood metabolites.

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