Notulae Scientia Biologicae, Volume (3), No (2), Year (2011-3) , Pages (104-107)

Title : ( Warm Stratification and Chemical Treatments Overcomes the Dormancy and Promotes Germination of Colchicum kotschyi Boiss Seeds under In Vitro Condition )

Authors: morteza alirezaie , Hossein Arouiee , Shamsali REZAZADEH , Mahmoud Shoor , Yahya selahvarzi , Navid Vahdati Mashhadian ,

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Colchicum kotschyi Boiss grows wildly in Iran. Propagation of this perennial geophyte plant takes a long time either by corm or seed. Seeds usually have a deep dormancy and take more than a year to germinate. Therefore in order to find out an effective method for this fact, a factorial experiment based on completely randomized design with four replicates was conducted to investigate the effects of warm stratification (no stratification and 8 weeks, 25°C) and different chemical priming treatments. Priming was done by: H2SO4 (0 and 50%) and GA3 at (0, 250, 500 and 1000 ppm) and KNO3 at (1 and 2%). Seeds were all cultured in a Potato Dextrose Agar media (1%) and put in a dark position to germination. The results showed that warm stratification priming alone, had a germination percentage of 28.7%. No germination was observed in control, H2SO4 and KNO3 treatments alone. Warm stratification along with GA3 significantly increased the germination percentage. Maximum germination percentage (70%) was obtained in warm stratification and GA3 (1000 ppm). It seems that the problem to germination in most of the Colchicum kotschyi seeds is probably due to a kind of morphophysiological dormancy which is broken by warm stratification along with GA3.

Keywords

, Colchicum kotschyi, geophyte, morphophysiological dormancy,
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@article{paperid:1021882,
author = {Alirezaie, Morteza and Arouiee, Hossein and Shamsali REZAZADEH and Shoor, Mahmoud and Selahvarzi, Yahya and Vahdati Mashhadian, Navid},
title = {Warm Stratification and Chemical Treatments Overcomes the Dormancy and Promotes Germination of Colchicum kotschyi Boiss Seeds under In Vitro Condition},
journal = {Notulae Scientia Biologicae},
year = {2011},
volume = {3},
number = {2},
month = {March},
issn = {2067-3205},
pages = {104--107},
numpages = {3},
keywords = {Colchicum kotschyi; geophyte; morphophysiological dormancy; priming},
}

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%0 Journal Article
%T Warm Stratification and Chemical Treatments Overcomes the Dormancy and Promotes Germination of Colchicum kotschyi Boiss Seeds under In Vitro Condition
%A Alirezaie, Morteza
%A Arouiee, Hossein
%A Shamsali REZAZADEH
%A Shoor, Mahmoud
%A Selahvarzi, Yahya
%A Vahdati Mashhadian, Navid
%J Notulae Scientia Biologicae
%@ 2067-3205
%D 2011

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