Objective: contaminated poultry meat has been identified as one of the principal foodborne sources of salmonella. PCR has become the potential of a powerful alternative in microbiological diagnostics due to its rapidity and accuracy. In this study a survey was carried out to determine the prevalence of salmonella spp. and its enteritidis serovar in broiler carcasses. Materials and Methods: a total of 100 samples, representing 10 broiler flocks, were taken from poultry carcasses which were prepared for packaging at a commercial broiler slaughtering facility in Mashhad using rinse test method. The presence of salmonella spp. And salmonella enteritidis was assessed by pre-enrichment, enrichment, selective and differential plating of centrifuged rinsed water. Then DNA was extracted and multiplex PCR performed by amplification of invA gene which is specific for detection of salmonella spp. And prot6 gene which is specific for detection of salmonella enteritidis. Results and Conclusion: In this study 14% of poultry carcasses were determined as contaminated with salmonella spp. And 6% as contaminated with a salmonalla enteritidis. It is pertinent to use m=PCR in order to provide a more accurate profile of the prevalence of salmonella spp. and slmonella enteritidis in broiler carcasses that could be considered as an appropriate alternative to conventional culture method.

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