This experiment was conducted to determine the effect of different sources of pectin feedstuffs on blood metabolites in early lactating Holstein cows. Eight primiparous early lactating Holstein cows (60 ± 23 d postpartum, weighing 530 ± 60 kg) were assigned into a duplicated 4 × 4 Latin square design with 4 3-wk periods. Cows were allocated into 4 diets whit 1)10% barley grain, 2)10% sugar beet pulp, 3)10% wheat bran and 4) 10% dried citrus pulp . Each experimental period was 21 d including 14 d adaptation period and 7 d collecting samples. Blood samples were collected from the jugular vein 2h after the morning feeding. Blood serum was collected after centrifuged at 1,500 × g for 20 min, frozen at – 40°C, and later analyzed for glucose, urea N, cholesterol, triglyceride, alkaline phosphate and albumin. Blood metabolites data and DMI are shown in Table 1.There were no differences (P > 0.05) on daily intake of DM (kg/d) (Table 1). Similarly, no treatments effects were observed blood metabolites (Table 1). These data suggest that, the addition of sources of pectin feedstuffs at 10% levels (dry matter basis) of the dairy cow ration instead of cereal grain; will decrease the cost of milk production without any negative effect on DMI and blood metabolites. Table 1. Blood metabolites of dairy cows Item Treatment T1 T2 T3 T4 SEM DMI (kg/day) 19.34 19.63 19.74 18.69 0.348 Glucose (mg/dL) 55.8 53.8 54.1 53.5 22.902 Blood urea nitrogen (mg/dL) 24.37 25.62 25.87 26.25 0.786 Cholesterol (mg/dL) 186.25 186.88 193.88 185.63 8.142 Triglyceride (mg/dL) 9.25 10.625 10.625 12.87 2.69 Alkaline phosphate (U/L) 68.25 75.5 69.25 71.87 4.212 Albumin (g/dL) 4.23 4.25 4.28 4.22 0.047

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