Enterohemorrhagic Escherichia coli O157:H7 (EHEC) is an important verotoxin producing E. coli (VTEC). It is associated with food and water borne infections. This study was designed to carefully examine the effects of four different factors on the growth of E. coli O157:H7 in the brain heart infusion broth. These factors included four concentrations of Carum copticum (Zenyan in Persian) essential oil (0, 0.015, 0.03 and 0.06%), with the major components of thymol (57.18%), p-cymene (22.55%), and γ-terpinene (13.07%), two incubation temperatures (35 and 25°C), three levels of pH (5, 6 and 7) and two inoculum size (103 and 105 cfu ml-1). The experiment was carried out in triplicate. Growth was monitored by visible turbidity during a 30-day period. To evaluate the effects of explanatory variable on time to detection of bacterial growth, parametric survival model based on the weibull distribution was used. All explanatory variables had significant association with time to detection (P < 0.05). The models accurately predicted the growth initiation and inhibition of E. coli O157: H7.

Keywords