Hydatidosis is considered to be an important economic and human public health problem in Iran, where a variety of animals act as intermediate hosts. There is limited information about the genotypes of Echinococcus granulosus in camels. In this study, PCR-RFLP system was established for rapid detection of Echinococcus granulosus genotype sensu strict (G1_G3) and E. granulosus genotype G6/7. For this aim a primer pair derived from mitochondrial internal transcribed spacer-1 (mtITS-1) was used which was able to amplify the corresponding gene segment in abovementioned organisms, resulting in a PCR product of 593 bp in length having organisms specific Bsh12361 restriction sites. Four hundred eight dromedaries examined from five Province in half Eastern of Iran. Seven hundred hydatid cysts were detected in 135 infected animals, from which 50 samples were selected randomly for molecular analysis. The molecular analysis showed that 27 cysts belonged to the G1 genotype and 23 cysts to the G6 genotype. PCR-RFLP revealed two different restriction patterns for both of genotypes. The nucleotide sequence analysis of PCR products confirmed the results of molecular. Additionally, the nucleotide sequence analysis of mitochondrial cytochrome c oxidase subunit 1 (cox1) genes confirmed the genotype determination. As previously reported, G1 is the most strain that infect humans in the entire world; accordingly, camel's role in the epidemiology of this strain and its transmission to humans, should be considered in controlled studies.

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