In considering the interaction between microorganisms and insecticides, some certain effects may become significant. These effects involve the potential inhibition or possible germicidal action of insecticides on rumen microbes. Proper functioning of these microorganisms is essential, since they supply approximately 50 to 70% of the energy requirements of ruminants. Any deleterious influence, even of a temporary nature, could have a profound effect on the bacterial activity and subsequently upon the host animal. So a study to investigate the effects of different levels (0, 0.7, 2.8 and 5.6 mg) of phosalone as an insecticide with different levels of calcium bentonite (0 and 100 mg) on in vitro dry matter disappearance was carried out in the farm of animal science in Ferdowsi University of Mashhad, Iran in 2012. The study indicated that all the levels of phosalone had not any significant effect on in situ dry matter disappearance (ISDMD),only the effect of phosalone was significant after 24h incubation (p<0.05). There was a significant effect (P<0.01) for calcium bentonite for effective dry matter digestibility (EDMD), the rapidly soluble fraction (a) and the potentially degradable fraction (b). In this experiment calcium bentonite is tested as toxin binder for decreasing the probably deleterious effects of phosalone on rumen degradability of dry matter.

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