International Multidisciplinary Cancer Congress , 2012-09-04

Title : ( Evaluation of Oct4 Expression Using p-Oct4-GFP Reporter Construct in Human Embryonic Kidney (HEK293T) Cell line. )

Authors: Mina Shahriyari , zahra hejazi , Ahmad Reza Bahrami , Maryam Moghaddam Matin , Alikabar haddad Mashadrizeh , Moein Farshchian ,

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Background: Oct4, a POU family transcription factor, is one of the most critical genes during reprogramming and its expression is significantly correlated with stemness state of cells.In this study we suggested a vector which express Green fluorescent protein (GFP) under Oct4 promoter for analysing Oct4 expression in the competent cells. Material & Method: We used a genetic construct which promote GFP under Oct4 gene promoter and transfected the HEK-293T cells using calcium phosphate method.GFP expression was assessed by Fluorescent microscopy 12-24 hrs after transfection. Results: We managed to produce GFP positive,about 40-50%, cells post transfection which highlight the activity of Oct4 gene in the HEK-293T cells. Discussion: Activity of Oct4 in cell lines like HEK is an intriguing issue which precise role of it remain to be clarified. Taken together, Oct4 expression might be used as a reliable marker to reveal the tumoral stage in cancerous cells, and methods that evaluate Oct4 expression of cells could be used as a prognosis marker which correlate with cliniopathological signs of cancers.

Keywords

, HEK293T cells, Transfection, Oct4
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@inproceedings{paperid:1033499,
author = {Shahriyari, Mina and Hejazi, Zahra and Bahrami, Ahmad Reza and Moghaddam Matin, Maryam and Haddad Mashadrizeh, Alikabar and Farshchian, Moein},
title = {Evaluation of Oct4 Expression Using p-Oct4-GFP Reporter Construct in Human Embryonic Kidney (HEK293T) Cell line.},
booktitle = {International Multidisciplinary Cancer Congress},
year = {2012},
location = {مشهد, IRAN},
keywords = {HEK293T cells;Transfection;Oct4 expression},
}

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%0 Conference Proceedings
%T Evaluation of Oct4 Expression Using p-Oct4-GFP Reporter Construct in Human Embryonic Kidney (HEK293T) Cell line.
%A Shahriyari, Mina
%A Hejazi, Zahra
%A Bahrami, Ahmad Reza
%A Moghaddam Matin, Maryam
%A Haddad Mashadrizeh, Alikabar
%A Farshchian, Moein
%J International Multidisciplinary Cancer Congress
%D 2012

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