Objectives: The purpose of this study was to determine the effects of using caffeine and trans-cinammaldehyde on serum glucose, ALT/SGPT, AST/SGOT and cholesterol level in male broilers chicks. Materials and Methods: This experiment was conducted in a completely randomized design with 350 of broiler (Ross-308) in 7 treatments and 5 replicate (with 10 hens in each replicate) from 1 to 21 days and included: 1) control group without any additives, 2) 0.5% caffeine powder 3) 1% caffeine powder 4) 2% caffeine powder, 5) 0.05% trans-cinammaldehyde, 6) 0.1% transcinammaldehyde and 7) 0.15% trans-cinammaldehyde. At 21d day of the experiment, the blood vessels of the neck for glucose, ALT, AST and cholesterol level measurements of each replicate were slaughtered as a chicken\\\\\\\'s cervical dislocation method. Bloods serums were separated and send to the laboratory to do next. Serum glucose, ALT, AST and cholesterol were analyzed with Auto Analyzer (Bio Systems S. A. – Costa Brava 30, 08030 Barcelona, Spain). All data were analyzed by the analysis of variance (ANOVA) option GLM of SAS/STAT software (SAS Institute, 2001) and when treatment means were significant (P < 0.05), Tukey’s multiple range tests was used to compare means. Results and Conclusion: The result of this research showed that different level of caffeine and Trans- cinnamaldehyde had no significant effect on serum ALT, AST and cholesterol level. In the current experiment Trans- cinnamaldehyde had no significant effect on serum glucose. However, increasing caffeine content from 0.1 to 0.2 % significantly reduced plasma glucose. Based on the data from this experiment, it was concluded that there is no different in usage of 3 level of Trans- Cinammaldehyde, and 2 level of caffeine (0.5 and 0.1%) in poultry diets. Keywords: Broilers, Caffeine, Trans-Cinammaldehyde, Blood parameters.

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