Objectives: This study was conducted to evaluate the serotonin and corticostrone levels on day-old male broiler chicks fed caffeine and trans-cinammaldehyde. Materials and Methods: This experiment was conducted in a completely randomized design with 350 of broiler (Ross-308) in 7 treatments and 5 replicate (with 10 hens in each replicate) from 1 to 21 days and included: 1) control group without using any additives, 2) 0.5% caffeine powder 3) 1% caffeine powder 4) 2% caffeine powder, 5) 0.05% trans-cinammaldehyde, 6) 0.1% transcinammaldehyde and 7) 0.15% trans-cinammaldehyde. At 21d day of the experiment, the blood vessels of the neck for serotonin and corticostrone measurements of each replicate were slaughtered as a chicken\\\\\\\'s cervical dislocation method. Bloods serums were separated and send to the laboratory to do next. Serotonin was determined with Eliza kit (Labor Diagnostika Nord Gmbh & Co. KG) and cortocostrone was analyzed with EIACS kit (Assay Designs Inc., Ann Arbor, MI). All data were analyzed by the analysis of variance (ANOVA) option GLM of SAS/STAT software (SAS Institute, 2001) and when treatment means were significant (P < 0.05), Tukey’s multiple range tests was used to compare means. Results and Conclusion: Final results of this study have shown that with increasing level of Transcinnamaldehyde and caffeine serotonin and corticostrone numerically decreased, but there was no significant difference in serum level of serotonin and corticostrone. It was concluded that there was no significant effects on serotonin and corticostrone level of broiler chicks fed different caffeine and Trans-cinnamaldehyde levels in the diets.

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