Objectives: The objective of this study was to evaluate the effects of a caffeine and trans-cinammaldehyde on hematological parameters of broiler chickens. Materials and Methods: A total of 350 Ross one day-old broiler chicks were grown over 21 days. Chicks were wing-banded, individually weighed and randomly allocated into five equally major groups each of five replicates. Chicks of group 1 (control group) were fed the starter and grower diets that were not supplemented. The chicks of groups 2, 3, and 4 were fed control starter and grower diets plus 0.5%, 1% and 2% of caffeine, 5, 6 and 7 were fed 0.05%, 0.1% and 1.5% transcinammaldehyde, respectively for 21 days. Blood samples were collected in K3EDTA tubes. Each sample was coded and offered for manual microscopic count. For the relative microscopic differential count (M-Diff), blood smears were made and stained with May-Gru¨nwald-Giemsa stain. Heterophils, lymphocytes, monocyte, basophile, eosinophil, hemoglobin and the H: L ratios were determined using the stained-slide method described by Gross and Siegel (1983). All data were analyzed by the analysis of variance (ANOVA) option GLM of SAS/STAT software (SAS Institute, 2001) and when treatment means were significant (P < 0.05), Tukey’s multiple range tests was used to compare means. Results and Conclusion: The results of this study showed that 2% caffeine significantly decreased hemoglobin and hematocrite level in broilers chicks’ serum. Although different level of Trans –cinnamldehyde, 0.5 and 1% caffeine had no significant effects on blood cell numerations. Based on the results of this study, it could be advised to don’t supplement broiler feed with 2% pure caffeine.

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