In vitro propagation of rose has played a very important role in rapid multiplication of species with desirable traits and production of healthy and disease-free plants. Micropropagation using nodal segments of Rosa canina under different combinations of BAP, GA 3 and NAA on (Ms and Vs), medium was investigated. The results showed that the highest shoot proliferation was obtained on Vs medium containing 8 µM BAP without any GA 3 and NAA. Furthermore the highest root regeneration obtained in half strength VS medium. The present investigation recommended a practicable in vitro plant protocol for R. canina as an important step for successful implementation of biotechnological techniques for rose improvement in Iran.

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