3rd national congress on medicinal plants , 2014-05-14

Title : ( Total phenol and flavonoid content and antioxidant activity of methanolic extract from Perovskia abrotanoides Karel at different stages of plant growth and development )

Authors: roya ebrahimifakhr , Parvaneh Abrishamchi , Maryam Moghaddam Matin , جواد اصیلی , Maryam Varasteh kojourian ,

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Perovskia abrotanoides Karel is an Iranian medicinal plant belonging to the Lamiaceae family. Different biologocal properties are mentioned for the plants of this genus including antibacterial, antioxidant, cytotoxic activities and apoptosis induction. Present study was carried out to evaluate the amount of phenolic compounds and antioxidant properties of metanolic extracts of P. abrotanoides Karel leaves at vegetative and flowering stages. Aerial parts of the plant, were collected from Kalat in northeast of Khorassan Razavi province of Iran. Phenolic compounds were extracted by sonication method. Total phenol and flavonoid contents of all extracts were obtained by aluminum chloride[1] and Folin-Ciocalteu[2] colorimetric methods, respectively. Antioxidant activities of extracts were measured by β-carotene bleaching (BCB) assay[3]. Phenolics content and antioxidant activity of leaves at vegetative stage, were significantly more than the other stage. The amount of total phenol and flavonoids of leaves at vegetative stage were estimated as 4.347 ± 0.067 g gallic acid and 1.063 ± 0.043 g quercetin per 100 g dry weight, respectively. At vegetative stage, leaves extract had the best performance in BCB assay (IC50 = 0.267 ± 0.03 mg/ml); compared with flowering stage (IC50 = 0.516 ± 0.06 mg/ml). A positive correlation was observed between the total phenol and flavonoid concentrations, and antioxidant activity of extracts in BCB assay (R2=0.89 & 0.90, respectively). it may represent the the role of phenolic and flavonoid compounds of the plant in its antioxidant potential.

Keywords

, Perovskia abrotanoides Karel, total phenol, flavonoid, antioxidant
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@inproceedings{paperid:1044896,
author = {Ebrahimifakhr, Roya and Abrishamchi, Parvaneh and Moghaddam Matin, Maryam and جواد اصیلی and Varasteh Kojourian, Maryam},
title = {Total phenol and flavonoid content and antioxidant activity of methanolic extract from Perovskia abrotanoides Karel at different stages of plant growth and development},
booktitle = {3rd national congress on medicinal plants},
year = {2014},
location = {مشهد, IRAN},
keywords = {Perovskia abrotanoides Karel; total phenol; flavonoid; antioxidant activity},
}

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%0 Conference Proceedings
%T Total phenol and flavonoid content and antioxidant activity of methanolic extract from Perovskia abrotanoides Karel at different stages of plant growth and development
%A Ebrahimifakhr, Roya
%A Abrishamchi, Parvaneh
%A Moghaddam Matin, Maryam
%A جواد اصیلی
%A Varasteh Kojourian, Maryam
%J 3rd national congress on medicinal plants
%D 2014

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