Diosgenin have various effects, such as a hypocholesterolemic action, an antioxidant activity and anticancer effects against a wide variety of tumor cells. This paper studies the effects of different media and various concentrations of NAA,2,4-D and Kin hormones on the induction, callus growth and level of diosgenin in callus culture of Trigonella foenumgraecum leaf. The results showed that the MS medium supplement with 1.5 mg/l 2,4-D and 0.5 mg/l Kin hormones is the best medium for callus induction and growth and diosgenin accumulation. The amount of diosgenin detected in leaf callus at 60 days was more than to 20, 40 and 80 days after culture. The data showed that 2,4-D hormone compared with NAA hormone caused 32% increase in diosgenin content.

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