Dog rose (Rosa canina L.) is one of the most important ornamental and medicinal plants which are used as a rootstock for ornamental roses such as Rosa hybrid and Rosa floribunda. In vitro propagation of rose has a very important role in rapid multiplication of species with desirable traits and in production of healthy and disease-free plants. Micropropagation of Rosa canina L. was revised, using its nodal segments under different combinations of BAP (0, 0.5, 1, 1.5 and 2 mgl⁻1), GA3 (0 and 0.5 mgl⁻1) and NAA (0 and 0.5 mgl⁻1) on Murashige and Skoog (MS) and Van der Salm medium (VS) in proliferation stage and using different combinations of NAA and IBA (0, 0.3, 0.6 and 0.9 mgl⁻1) and ½ VS medium in rooting stage. The highest shoot proliferation was obtained on VS medium containing 2 mgl⁻1 BAP. Furthermore, the highest root induction obtained in ½ VS containing 0.6– 0.9 mgl⁻1 of NAA or IBA. The present study presents an in vitro protocol for R. canina.

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