Cellular Reprogramming, ( ISI ), Volume (18), No (5), Year (2016-10) , Pages (333-343)

Title : ( Dedifferentiation Effects of Rabbit Regenerating Tissue on Partially Differentiated Cells )

Authors: mahdi mirahmadi , saeideh nakhaei rad , Maryam Moghaddam Matin , Mina Shahriyari , Morvarid Saeinasab , zahra mahmoodi , Azadeh Haghighitalab , Nasser Mahdavi SHahri , Ahmad Reza Bahrami ,

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Cell Stemness can be achieved by various reprogramming techniques namely, somatic cell nuclear transfer, cell fusion, cell extracts, and introduction of transcription factors from which induced pluripotent stem cells (iPSCs) are obtained. iPSCs are valuable cell sources for drug screening and human disease modeling. Alternatives to virus-based introduction of transcription factors include application of DNA-free methods and introduction of chemically defined culturing conditions. However, the possibility of tumor development is still a hurdle. By taking advantage of NTERA-2 cells, a human embryonal carcinoma cell line, we obtained partially differentiated cells and examined the dedifferentiation capacity of regenerative tissue from rabbit ears. Results indicated that treatment of partially differentiated NTERA-2 cells with the regenerating tissue-conditioned medium (CM) induced expression of key pluripotency markers as examined by real-time polymerase chain reaction, flow cytometry, and immunocytochemistry techniques. In this study, it is reported for the first time that the CM obtained from rabbit regenerating tissue contains dedifferentiation factors, taking cells back to the pluripotency. This system could be a simple and efficient way to reprogram the differentiated cells and generate iPSCs for clinical applications as this system is not accompanied by any viral vector, and reprograms the cells within 10 days of treatment. The results may convince the genomic experts to study the unknown signaling pathways involved in the dedifferentiation by regenerating tissue-CM to authenticate the reprogramming model.

Keywords

, PLURIPOTENT STEM, CELLS; HUMAN SOMATIC, CELLS; DEFINED FACTORS; LIMB REGENERATION; HUMAN FIBROBLASTS; SURFACE, ANTIGENS; SMALL, MOLECULE; SELF, RENEWAL; EAR HOLES; EC
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@article{paperid:1060200,
author = {Mirahmadi, Mahdi and Nakhaei Rad, Saeideh and Moghaddam Matin, Maryam and Shahriyari, Mina and Saeinasab, Morvarid and Mahmoodi, Zahra and Haghighitalab, Azadeh and Mahdavi SHahri, Nasser and Bahrami, Ahmad Reza},
title = {Dedifferentiation Effects of Rabbit Regenerating Tissue on Partially Differentiated Cells},
journal = {Cellular Reprogramming},
year = {2016},
volume = {18},
number = {5},
month = {October},
issn = {2152-4971},
pages = {333--343},
numpages = {10},
keywords = {PLURIPOTENT STEM-CELLS; HUMAN SOMATIC-CELLS; DEFINED FACTORS; LIMB REGENERATION; HUMAN FIBROBLASTS; SURFACE-ANTIGENS; SMALL-MOLECULE; SELF-RENEWAL; EAR HOLES; EC CELLS},
}

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%0 Journal Article
%T Dedifferentiation Effects of Rabbit Regenerating Tissue on Partially Differentiated Cells
%A Mirahmadi, Mahdi
%A Nakhaei Rad, Saeideh
%A Moghaddam Matin, Maryam
%A Shahriyari, Mina
%A Saeinasab, Morvarid
%A Mahmoodi, Zahra
%A Haghighitalab, Azadeh
%A Mahdavi SHahri, Nasser
%A Bahrami, Ahmad Reza
%J Cellular Reprogramming
%@ 2152-4971
%D 2016

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