Title : ( Recombinant lentivirus expression vector for insulin-like growth factor (IGF-1) gene delivery )
Authors: Zahra Roudbari , Mohammadreza Nassiri , Mojtaba Tahmoorespur , Aliakbar Haddad-Mashadrizeh , Ali Javadmanesh ,
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Abstract
DNA IGF1 was used as transfer vector to produce recombinant IGF1 in mammalian cells. Transfer and control vector were co-transfected into HEK293T cell line with helper plasmids to produce recombinant lentivirus. HEK293T cells with lentivirus particles were then transducted to produce recombinant HEK293T cell line. In-vitro mRNA and protein overexpression of IGF-1 were determined by RT-qPCR and western blotting. The method suggests that a constructed recombinant lentivirus vector could be used to introduce IGF-1 genes into mammalian cells.
Keywords
, IGF1, recombinant lentivirus, growth trait, mammalian cells
@article{paperid:1060688,
author = {Roudbari, Zahra and Nassiri, Mohammadreza and Tahmoorespur, Mojtaba and Haddad-Mashadrizeh, Aliakbar and Javadmanesh, Ali},
title = {Recombinant lentivirus expression vector for insulin-like growth factor (IGF-1) gene delivery},
journal = {Online Journal of Veterinary Research},
year = {2016},
volume = {20},
number = {8},
month = {October},
issn = {1328-925X},
pages = {563--570},
numpages = {7},
keywords = {IGF1; recombinant lentivirus; growth trait; mammalian cells},
}
%0 Journal Article
%T Recombinant lentivirus expression vector for insulin-like growth factor (IGF-1) gene delivery
%A Roudbari, Zahra
%A Nassiri, Mohammadreza
%A Tahmoorespur, Mojtaba
%A Haddad-Mashadrizeh, Aliakbar
%A Javadmanesh, Ali
%J Online Journal of Veterinary Research
%@ 1328-925X
%D 2016
