Abstract This study was conducted with two consequential experiments. The first experiment was conducted to investigate the effect of magnetized water and particle size on in-vitro limestone solubility. Three particle sizes including small particles, less than 0.125 mm; large particles, 2-4 mm and normal mix were tested. Magnetized water was generated by magnetizer of 0.65 Tesla magnetic fields. Scanning Electron Microscopy (SEM) was used to study the morphology of limestone crystals after dissolution in magnetized and tap water. Limestone solubility was measured by 0.2 N HCl solution. The second in- vivo experiment was conducted to study the effect of magnetized water and dietary calcium on breaking strength of eggs. Three dietary levels of calcium and phosphorus (normal, 10 and 20% reduced Ca and Available phosphorous, avail. P) and two types of water (tap water and magnetized water) were used in Hy-line laying hens. Breaking strength of the safe and sound eggs were measured with an instron testing machine. The solubility of large limestone particles was less than those of small particles. Magnetically treated water did not change in-vitro limestone solubility but changed the morphology of limestone crystals. Precipitated limestone crystals in magnetized water tended to be larger and more uniform in size than those in tap water. Reducing dietary levels of Ca and P had no significant effect of egg breaking strength. Magnetized water was able to numerically increase strength of the eggs. Therefore, based on the SEM images and the observed changes in crystalline structure of dissolved precipitated limestone and observed changes in breaking strength of the eggs, it is hypothesized that magnetized water may have the potential to change the limestone availability and consequently egg strength in laying hens.

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