One of the most important mycotoxins which is produced by toxigenic strains of different Aspergillus spp. are aflatoxin B1 (AFB1). The aim of this 3-wk study was to evaluate the ability of sodium bentonite (NaB), Saccharomyces cerevisiae (yeast) and Lactobasillus rhamnosus strain GG (LAB) to alleviate the deleterious effects of AFB1 on intestinal morphology in broiler chicks. Three hundred and twenty 1-d-old as hatched broilers (Ross 308) were maintained in the cage litter and allowed ad libitum access to feed and water. A completely randomized design was used with 5 replicate pens of 8 chicks assigned to each of 8 dietary treatments from hatch to 21 d. Dietary treatments included: 1) basal diet (BD) with no AFB1 or any binder, 2) contaminated diet (CD) with AFB1 (2 mg/kg of feed), 3, 4, 5) BD supplemented with NaB (0.5%), yeast and LAB 6, 7, 8) CD supplemented with NaB (0.5%), yeast and LAB. Morphological characteristics were measured at different segments of intestinal lumen. AFB1 decreased the villus height (700.05) in jejunum and villus height (2.71) to crypt depth ratio (3.68) in jejunum and ilium in comparison with control (P<0.05). However, addition of three binders could partially increase these features. In conclusion, it is suggested by this study that in feed contaminated with AFB1, the use of sodium bentonite, yeast and LAB is an efficient strategy to reduce the adverse effects of aflatoxicosis in broiler chicks.

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